Robert L. Moritz, PhD.

Institute for Systems Biology, Seattle, WA, U.S.A.

Technology advances in mass spectrometry have provided unprecedented levels of protein identification and quantitation across a multitude of organisms, including human. To capitalize on these advances in comprehensive proteome interrogation, new tools, methods and bioinformatics approaches are required to propel these advances to broad and routine usage. The democratization of these resources will enable these tools to be routinely used and enable biologically meaningful data to be collected, analyzed and shared. To support broad dissemination of these enabling tools and resources, we have developed new systems and repositories and integrated these developments into our existing and new suites of bioinformatics software and repositories. I will discuss these tools that are capable of generating complete, reproducible and quantitatively accurate proteome datasets. Examples of these include our developments in targeted proteomics utilizing SRM and SWATH-MS and applications to quantitative biomarker discovery.