III IBSB – Enzymes for Biorenewables

Enzymes for Biorenewables is part of a workshop series promoted by the Brazilian Biorenewables National Laboratory, from the Brazilian Center for Research in Energy and Materials (LNBR/CNPEM) in order to address the main challenges of Biotechnology. CNPEM is a non-profit private organization under the supervision of the Brazilian Ministry of Science, Technology and Innovations (MCTI).

In this edition, the most recent research res.ults regarding enzyme prospection and design, carbohydrate-active enzymes, and redox enzymes mechanism, as well as the development of microbial platforms for the production of industrial enzymes will be discussed. This event is expected to create a welcoming space for discussions and networking among the participants, which will comprise undergraduate and graduate students, professionals, professors, and researchers, working in the Biotechnology field in Brazil. The III IBSB aims to promote a forum to discuss key issues at the state of the art for industrial enzymes development, by disseminating researches developed in Brazil and encouraging a collaborative environment. The event will be hosted as a fully online meeting due to the COVID-19 pandemic.

Workshop Sessions
  • Microbiomics for Enzyme Discovery
  • Enzyme Mechanisms
  • Redox Enzymes
  • Fungal Platforms for Enzyme Production
Description Date
Registration Period April 19th to May 21st, 2021
Notification of Acceptance May 25th
III IBSB Workshop Day 1 June 7th – 9 am to 1 pm
III IBSB Workshop Day 2 June 8th – 9 am to 1 pm

Undergraduate and graduate students, researchers, and professionals with an interest in industrial enzymes working in any area of Biotechnology.

The Workshop will be free and conducted online, in a platform to be defined and communicated in due course. The slides will be presented in English, but the lectures will be conducted in Portuguese.

To allow interaction among participants, the event will be limited to an audience of up to 400 attendees (due to high demand, the organizing committee opted to double the audience capacity previously planned for 200 participants). Registrants must be involved with Biotechnology (including, for example, enzymology, structural biology, microbiology, bioprocess, and omics) and will be selected according to the order of their registration and proximity of their area of expertise to the scope of the event. All selected participants will be communicated via e-mail.


Microbiomics for Enzyme Discovery
Speaker/Activity Title Time
Participant log-in Participant log-in 8h15 – 9h
Mário Murakami (LNBR/CNPEM) Opening 9h – 9h10
João C. Setubal (Instituto de Química, USP) The Composting Microbiome 9h10 – 9h30
Gabriela Persinoti (LNBR/CNPEM) Integrated multi-omics of the capybara gut microbiome reveal unprecedented enzymatic strategies for plant polysaccharides breakdown 9h30 – 9h50
André Lima (UNIVALI) Enzyme prospection from South Atlantic deep-sea bacteria 9h50 – 10h10
Anete Pereira de Souza (Instituto de Biologia, UNICAMP, Campinas, SP) Bioprospecting of regulators and enzymes associated with cellulose degradation in Trichoderma harzianum by integrative genomics 10h10 – 10h30
Q&A with Speakers Q&A 10h30 – 10h50
Break Break 10h50 – 11h10
Enzyme Mechanisms
Speaker/Activity Title Time
Munir S. Skaf (Instituto de Química, UNICAMP, Campinas, SP) Glucopyranose ring opening by a glycoside hydrolase: Is it a viable reaction pathway? 11h10 – 11h30
Mariana Morais (LNBR, CNPEM) Combining experimental and in silico approaches to reveal the molecular mechanisms of xylanolytic glycoside hydrolases 11h30 – 11h50
Richard J. Ward (Departamento de Química, USP, Ribeirão Preto, SP) Designing Enzymes for the Bioeconomy: Improved Biocatalysts for Lignocellulose Breakdown 11h50 – 12h10
Vitor Leite (Instituto de Biociências, Letras e Ciências Exatas – IBILCE, UNESP, São José do Rio Preto, SP) Protein engineering by optimization of electrostatic interactions 12h10 – 12h30
Q&A with Speakers Q&A 12h30 – 12h50
Closing Remarks 1st day Closing Remarks 12h50 – 13h
Redox Enzymes
Speaker/Activity Title Time
Participant log-in Participant log-in 8h15 – 9h
To be defined (TBD) Opening 9h – 9h10
Fernando Segato (Escola de Engenhara de Lorena, USP, Lorena, SP) Functional studies of lytic polysaccharide monooxygenases identified in secretomes of filamentous fungi cultivated in sugarcane bagasse 9h10 – 9h30
Leticia Zanphorlin (LNBR/CNPEM) Decarboxylation mechanisms to produce drop-in chemicals from fatty acids by P450 peroxygenases 9h30 – 9h50
Marco A. Kadowaki (Université Libre de Bruxelles, École Interfacultaire de Bioingénieurs, Bruxelas, Bélgica) Probing the oxidative activity of lytic polysaccharide monooxygenases (LPMOs) with protein-film voltammetry 9h50 – 10h10
João Paulo Cairo (Instituto de Biologia, UNICAMP, Campinas, SP) The role of redox enzymes in lignocellulose digestion by termites and their biotechnological applications 10h10 – 10h30
Q&A with Speakers Q&A 10h30 – 10h50
Break Break 10h50 – 11h10
Fungal Platforms for Enzyme Production
Speaker/Activity Title Time
Roberto da Silva (USP, Ribeirão Preto, SP) The black box of transcriptional factors to improve cellulases production in Trichoderma reesei 11h10 – 11h30
Mario Murakami (LNBR/CNPEM) Rational design of a fungal platform for enzyme production under industrially-relevant conditions 11h30 – 11h50
André Damásio (Instituto de Biologia, UNICAMP, Campinas, SP) The knockout of lytic polysaccharide monooxygenases affects Aspergillus nidulans secretome and growth on plant biomass 11h50 – 12h10
Marli Camassola (Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS) Once upon a time… the evolution of enzyme cocktails for lignocellulosic treatment in biorefineries: the history of Penicillium echinulatum and enzyme production strategies 12h10 – 12h30
Q&A with Speakers Q&A 12h30 – 12h50
Closing Remarks 2nd day Closing Remarks 12h50 – 13h