II IBSB – Sustainable Chemicals and Fuels Through Synthetic Biology

Sustainable Chemicals and Fuels Through Synthetic Biology
May 28 – 30th, 2019 | LNBR/CNPEM

The continued use of fossil fuels is no longer tenable. A finite resource, their extraction, processing and exploitation results in environmental pollution and increased greenhouse gas emissions. The challenge facing global societies is to identify sustainable and cleaner processes for chemical, fuel and food production, while at the same time reducing GHG emissions, in particular CO2. Biological routes offer the most promising alternative where, to avoid conflict with the food chain, attention largely focuses on using waste, lignocellulosic biomass as the feedstock. However, its recalcitrance to deconstruction is making the development of economic processes extremely challenging.

Moreover, all currently used commercial, fermentative processes are limited to the production of relatively few chemicals and fuels and all result in the net production of CO2. In the EU, for example, alcoholic fermentations annually produce 6-8 Mtons of CO2. Synthetic Biology has a major role to play in meeting current challenges through the engineering of microbial strains (chassis) that are better able to directly convert lignocellulosic biomass, or derivative feedstocks such as sugar-rich hydrolysates or synthesis gas, into a more comprehensive array of products in processes that minimize, or even eliminate, CO2 production.

Our objective is to explore the numerous options available, ranging from the:

  • microbial chassis available for manufacturing processes;
  • most attractive chemical and fuel options;
  • consideration of the most effective lignocellulose-derived feedstock identity of the most efficient strategy for minimizing CO2 production;
  • the relative merits of the use of monoculture or synthetic communities.


Session Time Speaker and Lecture Institution
11:30 – 12:00 Registration
12:00 – 12:10 Welcoming Remarks
1 – Main participating research institutes (Nigel Minton) 12:10 – 12:20 Eduardo do Couto e Silva CNPEM/LNBR
12:20 – 12:30 Nigel Minton SBRC Nottingham
12:30 – 12:40 Alberto Acevedo INTA
12:40 – 12:50 Simon McQueen Mason CNAP
12:50 – 13:00 David Bryant IBERS
Lunch 13:00 – 14:30 Lunch
2- Conversion of Biomass Feedstocks (Klaus Winzer) 14:30 – 14:50 Otavio Cavalett: Lignocellulosic feedstocks for bioenergy in Brazil CNPEM/LNBR
14:50 – 15:10 Leo Gomez: Feedstock and processes for lignocellulosic biorefineries  Univeristy of York
15:10 – 15:30 Alberto Acevedo: Lignocellulosic feedstocks for bioenergy in Argentina INTA
15:30 – 15:50 Martin Holm: Biomass to sugar without enzymes Nova Pangea
Coffee break 15:50 – 16:20 Coffee break
2- Conversion of Biomass Feedstocks (Simon McQueen) 16:20 – 16:40 Sean Simpson: Benefits of synthesis gas (Syngas) fermentation Lanzatech
16:40 – 17:00 Ged Russell: Comparative sustainability of gas fermentation versus traditional sugar-based fermentation Vireol
Session Time Speaker and Lecture Institution
3 – Enzymes Discovery and Design (Mario Murakami) 09:00 – 09:20 Mario Murakami: Discovery and Design of CAZymes to Boost Biomass Deconstruction CNPEM/LNBR
09:20 – 09:40 Neil Bruce: Mining microbial communities for lignocellulose degrading enzymes CNAP
09:40 – 10:00 Igor Polikarpov: Optimizing enzymatic conversion of lignocellulosic biomass USP SP
10:00 – 10:20 Richard J. Ward: Chimerogenesis and molecular evolution USP RP
10:20 – 10:40 Annette Alcasabas: From Hit to Process: Enzyme Engineering at Johnson Matthey Johnson Matthey
Coffee break 10:40 – 11:00 Coffee break
4- Strain Engineering (Neil Bruce) 11:00 – 11:20 Nigel Minton: CRISPR/Cas9, TraDIS and orthogonal expression systems SBRC Nottingham
11:20 – 11:40 Telma Teixeira Franco:An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks Unicamp
11:40 – 12:00 Klaus Winzer: Metabolic engineering of Cupriavidus necator H16 for the sustainable production of 3-Hydroxyproprionic acid from CO2. SBRC Nottingham
12:00 – 12:20 Leandro Vieira: Yeast Engineering for 2G ethanol and other bioproducts CNPEM/LNBR
12:20 – 12:40 Katalin Kovacs: Engineered microbial factories for CO2 exploitation in an integrated waste treatment platform. SBRC Nottingham
12:40 – 13:00 Nadia Parachin: Use Of Non-Conventional Yeasts As A Chassis For Hyaluronic Acid Production University of Brasilia
Lunch 13:00 – 14:00 Lunch
4- Strain Engineering (Nigel Minton) 14:00 – 14:20 Andre Damasio: Filamentous fungi engineering Unicamp
14:20 – 14:40 Ying Zhang: Metabolic engineering of Geobacillus thermoglucosidasius bacilli for the production of chemicals and fuels. SBRC Nottingham
14:40 – 15:00 Philippe Soucaille: Increased butanol productivity through systems & synthetic biology. SBRC Nottingham
15:00 – 15:20 Michelle C. T. Batista: Novozymes’ Bio-Solutions for Cellulosic Ethanol Novozymes
15:20 – 15:40 Francisco Vassellucci GranBio
Tour of CNPEM 15:20 – 18:00 Tour of CNPEM (speakers only)  
Session Time Speaker and Lecture Institution
5 – Sugar Cane Waste Valorisation Projects (Alberto Acevedo) 09:00 – 09:20 Eduardo Couto: Bioethanol in Brazil CNPEM/LNBR
09:20 – 09:40 Juan Carlos Mirande: Production of bioethanol and electric power at La Florida sugarcane mill La Florida SA
09:40 – 10:00 David Bryant: Biorefining Value from Industrial Waste in India BIOREVIEW
10:00 – 10:20 Sanjay Nagarajan: Valorising Waste from Indian Sugar Cane and Associated Industries via Innovations in Pre-treatment, Biotransformation and Process Intensification Queen’s University Belfast
10:20 – 10:40 Simon McQueen Mason: Reducing industrial waste from sugarcane processing in India University of York
Coffee break 10:40 – 11:00 Coffee break
6 – Funding Landscape (Eduardo Couto) 11:00 – 11:15 Colin Miles BBSRC UK
11:15 – 11:30 Carlos Henrique de Brito Cruz FAPESP Brazil
11:30 – 11:45 Jorge Guimarães EMBRAPII Brazil
11:45 – 12:00 Jorge Tezon CONICET Argentina
12:00 – 12:15 Rui Lopes British Consulate in São Paulo
12:15 – 12:30 Juan Carlos Mirande LA Florida SA
General discussions 12:30 – 12:45 General discussions
Lunch 13:30 – 14:30 Lunch