Description

The second edition of the SMX workshop will contemplate discussions about the latest methods of serial data collection, both in situ and in jato, as well as the challenges and opportunities of room temperature dynamics studies of enzymes in cristal form. Automation, ease of use and remote access will be important components of the MANACA operation, and we will present and discuss the features of the pipelines for data collection and treatment under commisioning for the beamline.

Coordinator: Ana Zeri (LNLS/CNPEM)
Co-chair: Rafael Guido (USP)

Program

Time Activity Invited Speaker
10:45 – 11:00 The MANACA beamline at Sirius, status report on strategies to be available to users. Ana Carolina Zeri (CNPEM)
11:10 – 12:30 MXCuBE3 – the future of the MXCuBE collaboration. Daniele de Sanctis (ESRF)
13:45-14:15 Status of the implementation of MXCuBE3 for the control of the Manaca beamline. Lais Pessine do Carmo (CNPEM)
14:15- 15:00 Sample Holders for serial crystallography at the Manaca beamline. Evandro Araujo (CNPEM)
15:00 – 16:10 Visit to the Manaca beamline and chat with the beamline staff and Daniele de Sanctis

Local: Mezzanine Room

Target Public

  • Structural biology researchers, graduate and undergraduate students working on MX data collection;
  • Research professionals interested in protein structure determination and ligand binding studies.

Topics

  • Serial Macromolecular Crystallography;
  • Room Temperature Protein crystallography;
  • MX Data collection and beamline control.

Invited Speakers Abstracts

Daniele De Sanctis: ESRF

Title: MXCuBE3 – the future of the MXCuBE collaboration

Abstract: MXCuBE3 (Macromolecular Xtallography Customized Beamline Environment version 3) is the latest generation of a beamline control software initially designed and developed at the ESRF to perform macromolecular crystallography experiments. The project has developed into an international collaboration that will soon include 11 partner institutes over three continents (ESRF, ALBA, BESSY HZB, DESSY, EMBL, Global Phasing, MAXIV, Soleil, Elettra, LNLS and NSRRC).

MXCuBE3 takes advantage of the recent development in web technologies to create an intuitive and user friendly application. The access to the application from any web browser further simplifies the operation and natively facilitates the execution of remote experiments. We will present the overall architecture and the user experience of MXCuBE3 application and its use at third generation X-ray sources. We will discuss the future plans of development that encompass the scientific needs of the MX user community in term of automation and serial crystallography experiments.

This is a satellite event to the 29th edition of the LNLS Annual Users Meeting (RAU).