Program

Mar-24 – Tomogram reconstruction

  •  09:00h – 11:00h – Course introduction and movie alignment and preprocessing tools (motionCor, IMOD, etomo)
  •  11:00h – 11:30h – Coffee Break
  •  11:30h – 13:00h – Tilt series alignment: fiducial and fiducialless approaches (IMOD, AreTomo)
  •  13:00h – 14:00h – Lunch
  •  14:00h – 15:30h – CTF estimation and correction (ctffind5, AreTomo, IMOD)
  •  15:30h – 16:00h – Coffee Break
  •  16:00h – 17:00h – 3D Reconstruction and denoising (tomo3d, cryocare)
  •  17:00h – 19:00h – Gathering

Mar-25 – Particle Picking methods

  •  09:00h – 11:00h – Particle identification for oriented particles like membranes proteins (dynamo, pyseg)
  •  11:00h – 11:30h – Coffee Break
  •  11:30h – 13:00h – Particle identification for oriented particles like membranes proteins (dynamo, pyseg)
  •  13:00h – 14:00h – Lunch
  •  14:00h – 15:30h – Particle identification for non-oriented particles (cryolo, template matching deep finder)
  •  15:30h – 16:00h – Coffee Break
  •  16:00h – 17:00h – Particle identification for non-oriented particles (cryolo, template matching deep finder)

Mar-26 – Subtomogram Averaging techniques

  •  09:00h – 11:00h – Subtomogram averaging with dynamo and EMAN
  •  11:00h – 11:30h – Coffee Break
  •  11:30h – 13:00h – Subtomogram averaging with Relion5
  •  13:00h – 14:00h – Lunch
  •  14:00h – 15:30h – Subtomogram averaging with Relion5
  •  15:30h – 16:00h – Coffee Break
  •  16:00h – 17:00h – Subtomogram averaging with Relion5

Mar-27 – Practicals and Demonstrations on Data Acquisition and Processing

  •  09:00h – 11:00h – Practical on Data Processing
  •  11:00h – 11:30h – Coffee Break
  •  11:30h – 13:00h – Practical on Data Processing
  •  13:00h – 14:00h – Lunch
  •  14:00h – 15:30h – Tomography Data Collection
  •  15:30h – 16:00h – Coffee Break
  •  16:00h – 17:00h – Tomography Data Collection
  •  17:30h – 20:30h – Dinner

Mar-28 – Visit to IFSC-USP: Cryo-FIB-SEM 

  •  08:30h – Departing from Hotel Matiz
  •  10:30h – Arrival at IFSC-USP
  •  10:30h – 11:00h – Coffee-Break
  •  11:00h – 12:30h – Visit to Cryo-EM Facility and demo of FIB-SEM
  •  12:30h – 14:30h – Open discussion with lunch
  •  14:30h – 15:00h – Closing
  •  15:00h – Departing from IFSC-USP
  •  17:00h – Arrival at Hotel Matiz

Speakers and Instructors

  • Jose Luis Vilas (National Center of Biotechnology – CSIC)
  • Jorge Jiménez de la Morena (National Center of Biotechnology – CSIC)
  • Douglas Miquita (CM-UFMG)
  • Gabriela Noske (LNNano/CNPEM)
  • Beatriz Borges (LNNano/CNPEM)
  • João Marcos Faria (LNNano/CNPEM)