Integrative Structural Biology meets Drug Discovery
Workshop for Early Career Scientists
19-23 May 2025, CNPEM, Campinas, Brazil

Start End Talk title
8:00 8:30 Arrival
8:30 9:00 Welcome & Aim of the workshop

Daniela Trivella and Rodrigo Portugal, CNPEM

9:00 9:30 Short presentation of workshop organisers/training team (5-7min) Daniela Trivella, CNPEM
Karina Alleva, CEBEM
Bahne Stechmann, EU-OPENSCREEN
Rodrigo Portugal, CNPEM
John Dolan, Instruct-ERIC
9:30 10:00 Introduction of participants
10:00 10:30 Coffee break (30’)
10:30 11:30 Session on Drug Discovery 
10:30 11:00 Overview of Drug Discovery & Development

Daniela Trivella, CNPEM 

11:00 11:30 Drug Discovery process & strategies

Rui Moreira, iMed University of Lisbon 

11:30 12:30 Session on Drug Discovery 
11:30 12:00 Target selection and validation

Katlin Massirer, CQMED UNICAMP 

12:00 12:30 Hit discovery options: Target-based (biochemical, biophysical, fragment screening) and phenotypic screening methods

Olga Genilloud, Fundación MEDINA 

12:30 13:00 Q&A session
13:00 14:00 Lunch (60’)
14:00 15:30 Session on Drug Discovery 
14:00 14:30 Assay development and quality control

Bahne Stechmann, EU-OPENSCREEN

14:30 15:00 In silico approaches to Drug Discovery

Floriano Silva, Fiocruz 

15:00 15:30 Structural biology in Drug Discovery

Lizbe Koekemoer, Centre for Medicines Discovery (CMD), University of Oxford

15:30 16:00 Coffee break (30’)
16:00 17:30 Session on Structural Biology 
16:00 16:30 Introduction to X-ray crystallography

Beatriz Guimarães, FIOCRUZ Paraná

16:30 17:00 Introduction to NMR (remote)

Enrico Rivera, CIRMMP University of Florence

17:00 17:30 Introduction to cryo-EM

José Maria Valpuesta, CNB-CSIC

17:30 18:30 Networking & Social gathering
Start End Talk title
8:00 8:30 Transfer to venue
8:30 10:00 Session on Chemical Libraries
8:30 9:00 Chemical libraries, annotation & compound management

Rui Moreira, iMed University of Lisbon 

9:00 9:30 Natural products in the 21th century

Olga Genilloud, Fundación MEDINA

9:30 10:00 Chemical probes, fragment libraries and new drug modalities

Rui Moreira, iMed University of Lisbon

10:00 10:30 Coffee break (30’)
10:00 11:30 Session on Screening and bioassays
10:30 11:00 Biochemical and biophysical assays

Daniela Trivella, CNPEM

11:00 11:30 Phenotypic assays and complex cell models

Olga Genilloud, Fundación MEDINA

11:30 13:00 Session on hit follow up
11:30 12:00 Hit-to-lead optimisation principles (importance of ADME-Tox in DD)

Marjorie Bruder, CNPEM

12:00 12:30 Introduction to lead optimization (compound optimization by chemical synthesis)

Marjorie Bruder, CNPEM

12:30 13:00 Q&A session
13:00 14:00 Lunch (60’)
14:00 15:30 Session on NMR
14:00 14:30 NMR – principles part I

Luzineide Tinoco, Federal University of Rio de Janeiro

14:30 15:00 NMR – principles part II

Luzineide Tinoco, Federal University of Rio de Janeiro

15:00 15:30 NMR – fragment screening

Luzineide Tinoco, Federal University of Rio de Janeiro

15:30 16:00 Coffee break (30’) 
16:00 18:00 Session on Protein Crystallography
16:00 16:30 Protein Crystallography – principles part I

Beatriz Guimarães, FIOCRUZ Paraná

16:30 17:00 Protein Crystallography – principles part II

André Ambrósio University of São Paulo

17:00 17:30 Protein Crystallography – fragment screening

Lizbe Koekemoer, Centre for Medicines Discovery (CMD), University of Oxford

17:30 18:00 Protein Crystallography – Case study: Manaca beamline Sirius-CNPEM

Andrey Nascimento, CNPEM

18:00 19:00 Transport to hotel
Start End Talk title
8:00 8:30 Transfer to venue
8:30 10:00 Session on cryo-EM
8:30 9:00
CryoEM – Principles of Data Processing
Rodrigo Portugal, CNPEM
9:00 9:30
CryoEM – Current challenges for cryo-EM at an industrial scale
Michael Saur, Astex
9:30 10:00
CryoEM – Combining Structural and Functional Data: The Many Controls of the Enzyme Tyrosine Hydroxylase
José Maria Valpuesta, CNB-CSIC
10:00 10:30 Coffee break (30’)
10:30 13:00 Session on integrative approaches
10:30 12:00
Accelerating biological research using AlphaFold 3 (remote)
Oleg Kovalevskiy, DeepMind
12:00 12:30 Helder Veras, CNPEM
12:30 13:00 Integrating structural methods to elucidate the activation mechanism of human glutaminases
Andre Ambrósio, University of São Paulo 
13:00 14:00 Lunch (60’)
14:00 14:45
Cryo-EM for FBDD/SBDD at Astex
Michael Saur, Astex
14:45 15:00 Closing (theoretical part)
15:00 18:00 Visit to CNPEM facilities
18:00 19:00 Transport to hotel
Start End Talk title
8:00 8:30 Transfer to venue
8:30 12:30 Experimental session #1
12:30 13:30 Lunch (60’)
13:30 17:30 Experimental session #2
17:30 18:00 Q&A Practical Part
18:00 19:00 Transport to hotel
Start End Talk title
8:00 8:30 Transfer to venue
8:30 12:30 Experimental session #3
12:30 13:30 Lunch (60’)
13:30 17:30 Experimental session #4
17:30 18:00 Closing remarks
18:00 19:00 Transport to hotel