I MANACÁ Workshop Schedule

Event location: Sirius Auditorium, at the CNPEM campus, in Campinas-SP

August 4

  • Day 1: Introduction and Fundamentals
Theoretical Schedule
Time Activity Speakers
08:30 – 09:00 Registration and check-in –
09:00 – 09:10 Opening and welcome Evandro Araújo (LNLS/CNPEM), Andrey Nascimento (LNLS/CNPEM
09:10 – 09:50 Introduction and fundamentals –
Introduction to Sirius and its applications in life sciences Mateus Cardoso (LNLS/CNPEM)
09:50 – 10:10 EDU – User Office Karen Miranda (EDU/CNPEM), Felipe Vital (EDU/CNPEM)
10:10 – 10:30 Coffee break –
10:30 – 11:25 Protein production and purification Daniel Trindade (LNBIO/CNPEM)
11:25 – 12:20 Biophysical methods for protein characterization Leticia Zanphorlin (LNBR/CNPEM)
12:20 – 13:30 Methods for protein crystallization Priscila Giuseppe (LNBR/CNPEM)
13:30 – 14:30 Lunch –
14:30 – 16:00 RoboLab – Automated Protein Crystallization Laboratory Celisa Tonoli (LNBIO/CNPEM) 
16:00 – 16:30 Coffee Break –

 

Practical Schedule
Time Activity Speakers
15:00 – 16:30 Practical session (1): sample preparation and crystallization Celisa Tonoli (LNBIO/CNPEM) + All
16:30 – 18:00 Practical session (2): sample preparation and crystallization Celisa Tonoli (LNBIO/CNPEM) + All

 

August 5

  • Day 2: Data collection
Theoretical Schedule
Time Activity Speaker(s)
08:30 – 09:45 Student research project presentations (case studies and discussion)  ~ 3 min per student
09:45 – 10:30 Data collection  –
Introduction to crystallography and X-ray diffraction (1)  Beatriz Guimarães (FIOCRUZ/PR), Cristina Nonato (USP/RP)
10:30 – 10:50 Coffee break  –
10:50 – 13:30 Introduction to crystallography and X-ray diffraction (2)  Beatriz Guimarães (FIOCRUZ/PR), Cristina Nonato (USP/RP)
13:30 – 14:30 Lunch  –
14:30 – 15:30 Data collection  –
Principles of data collection using synchrotron light  Priscila Giuseppe (LNBR/CNPEM)
15:30 – 16:10 Introduction to the MANACÁ beamline and facilities  Evandro Araújo (LNLS/CNPEM)
16:10 – 16:30 Coffee break  –

 

Practical Schedule
Time Activity Speaker(s)
16:30 – 18:00 Practical session: Data collection techniques Andrey Nascimento, Evandro Araújo, Igor Maldonado (LNLS/CNPEM) + All
18:00 – 19:30 Data collection with student-provided samples  Andrey Nascimento, Evandro Araújo, Igor Maldonado (LNLS/CNPEM)

August 6

  • Day 3: Data processing and analysis
Theoretical Schedule
Time Activity Speaker(s)
08:30 – 09:30 Overview of data processing methods  Andrey Nascimento (LNLS/CNPEM) 
09:30 – 10:30 Software tools for data analysis  Andrey Nascimento (LNLS/CNPEM) 
10:30 – 11:00 Coffee break  –
13:00 – 14:00 Lunch –
14:00 – 15:00 Discussion of results and troubleshooting (speakers and students)  All
15:00 – 16:00 Methods and approaches for ligand and fragment screening  Cristina Nonato (USP/RP)
16:00 – 16:30 Coffee break –
16:30 – 17:30 Application of ligand and fragment screening Thamires Froes (FIOCRUZ/BA)
17:30 – 17:40 Official photo All
17:40 – 20:00 Social gathering –

 

Practical Schedule
Time Activity Speaker(s)
11:00 – 13:00 Practical session: Data processing and interpretation Andrey Nascimento, Evandro Araújo, Igor Maldonado (LNLS/CNPEM) + All

 

August 7

  • Day 4: Applications and future directions
Theoretical Schedule
Time Activity  Speaker(s)
08:30 – 09:15 Applications  –
Methods and approaches for natural product screening  Raphael Meneghello (LNBIO/CNPEM) 
09:15 – 10:00 Application of natural product screening  Andrew Oliveira (LNBIO/CNPEM)
10:00 – 10:30 Coffee break  –
10:30 – 12:00 Planning future experiments at the MANACÁ beamline  –
12:00 – 13:00 Special topics  –
Use of dynamic light scattering to assess and optimize protein crystallization  Christian Betzel (University of Hamburg) 
13:00 – 14:00 Lunch  –
14:00 – 14:45 Structural analysis of membrane proteins  Valentin Borshchevskiy (Moscow Institute of Physics and Technology) 
14:45 – 15:30 Introduction to serial and time-resolved crystallography  Evandro Araújo (LNLS/CNPEM) 
15:30 – 16:15 Combining XFELs and synchrotrons to decipher structural mechanisms of proteins in crystallo  Sandra Mous (SLAC National Accelerator Laboratory) 
16:15 – 16:40 Coffee break  –
16:40 – 17:25 Integrative biology and synchrotron light for novel enzymes and mechanisms  Mariana Morais (LNBR/CNPEM) 
17:25 – 18:30 Final student presentations and project discussions  –
18:30 – 19:00 Evaluation and closing remarks  Evandro Araújo, Andrey Nascimento (LNLS/CNPEM) + All