Program

Minicourse on Basic Principles of Light Microscopy

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Márcio Mesquita

Sílvio Roberto Consonni

31st August 2015

14:00 - 15:30	Principles in fluorescence, widefield and confocal microscopy Márcio Mesquita
15:30 - 15:50	Coffee break
15:50 - 17:10	Confocal and Super-resolution microscopy Márcio Mesquita
17:10 - 18:00	Sample preparation and troubleshooting in microscopy Sílvio Roberto Consonni
18:00 -	Wrap-up

Workshop Theoretical Activities

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Kleber Gomes Franchini
Director of LNBio

Piero Bagnaresi

Ricardo Vilela

Rodrigo Madeiro

Ulf Schwarz

Claudio Sunkel

Ricardo Vilela

Ulf Schwarz

Glaucia Hajj

Hozana Andrade Castillo

José Xavier Neto

Claudio Sunkel

1st September 2015

09:00 - 09:30	Welcome Kleber Gomes Franchini Director of LNBio
09:30 - 10:15	Basics of Confocal Microscopy Piero Bagnaresi
10:15 - 11:00	Leica TCS SP8: The Real Multiuser Confocal Platform Ricardo Vilela
11:00 - 11:30	Coffee break
11:30 - 12:30	Imaging Brain Cells to Understand Neuropsychiatric Disorders Rodrigo Madeiro
12:30 - 13:30	Leica TCS SP8 STED 3X: New Dimensions in Superresolution Microscopy Ulf Schwarz
13:30 - 14:30	Lunch
14:30 - 15:30	Guided tour through CNPEM
15:30 - 16:30	STED: Sample preparation and applications Ulf Schwarz
16:30 - 17:30	Monitoring progression through mitosis and its role in preventing aneuploidy Claudio Sunkel
17:30 - 18:00	#1 Selected poster – Oral section
18:00 - 20:00	Poster Session and Cocktail
2nd September 2015

09:00 - 10:00	Correlative Light Electron Microscopy Ricardo Vilela
10:00 - 11:00	The vertical Turn: Digital Light Sheet Ulf Schwarz
11:00 - 11:30	Coffee break
11:30 - 12:30	The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles Glaucia Hajj
12:30 - 13:30	Retinoic acid signaling regulation: insights from an evolutionary conserved enhancer Hozana Andrade Castillo
13:30 - 14:30	Lunch
14:30 - 15:30	Guided tour through CNPEM
15:30 - 16:30	Origin and evolution of cardiac chambers José Xavier Neto
16:30 - 17:30	Polo kinase and its role in regulating microtubule-kinetochore interaction Claudio Sunkel
17:30 - 18:00	Poster Awards and Theoretical Activities Closing Cerimony

Workshop Practical Activities

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• Leica TCS SP8
• Off Line WorkStation – LAS X
• Leica DM5500B
• Leica M205A

3rd September 2015

09:30 - 11:00	Practical Session I (4 Groups) • Leica TCS SP8 • Off Line WorkStation – LAS X • Leica DM5500B • Leica M205A
11:00 - 11:30	Coffee break
11:30 - 13:00	Practical Session II
13:00 - 14:00	Lunch
14:00 - 15:30	Practical Session III
15:30 - 16:00	Coffee Break
16:00 - 17:30	Practical Session IV
4th September 2015

09:30 - 10:30	Hands on Session I (4 Groups) • Leica TCS SP8 • Off Line WorkStation – LAS X • Leica DM5500B • Leica M205A
10:30 - 11:30	Hands on Session II
11:30 - 12:00	Coffee break
12:00 - 13:00	Hand on Session III
13:00 - 14:30	Lunch
14:30 - 15:30	Hands on Session IV
15:30 - 16:00	Farewell