“I am Atílio Tomazini, a professor at the Institute of Physics of the University of São Paulo (USP), affiliated with the Department of Applied Physics. I hold a B.Sc. in Biomolecular Physics, an M.Sc. and a Ph.D. in Applied Physics, with consolidated experience in structural biology, protein crystallography, molecular biology, and experimental biophysics.
My current research focuses on protein–protein interactions between fasciculin (FAS) and acetylcholinesterase (AChE), emphasizing rational protein engineering, biophysical characterization, and structural analysis of biomolecular complexes. In parallel, I also conduct structural studies of luciferase enzymes, aiming to elucidate the mechanisms of bioluminescence and their applications in biotechnology and bioimaging.
I am a member of the Biological Systems Physics Group at IFUSP, coordinated by Prof. Rosangela Itri, where I participate in projects combining experimental and computational approaches for the characterization of macromolecules and their interactions. Throughout my career, I have collaborated and carried out experimental activities in leading laboratories, including the research groups of Igor Polikarpov, Sasha Popov, David Levin, Mário Murakami, Vadim Viviani, and Júlia Shifman, which have strengthened my background in crystallography, structural biochemistry, and spectroscopy.
I have been conducting research as a user of the Brazilian Synchrotron Light Laboratory (LNLS) and the European Synchrotron Radiation Facility (ESRF) since 2011, employing several techniques for structural characterization of proteins. At LNLS–Sirius, my studies have directly benefited from protein X-ray diffraction (MX) experiments at the Manacá beamline, small-angle X-ray scattering (SAXS) at the SAPUCAIA beamline, and circular dichroism (CD) at the CEDRO beamline. I emphasize the scientific and strategic relevance of these facilities for the continuation and expansion of structural studies of macromolecules and materials in solution, benefiting the entire Sirius user community.
Throughout my academic trajectory, I have sought to integrate physics, biology, and chemistry in the training of new researchers, supervising students at different levels and promoting the use of advanced techniques of structural characterization, particularly those based on synchrotron radiation. I believe that my experience in multidisciplinary and collaborative projects, both in Brazil and abroad, can contribute to strengthening the dialogue between different fields of knowledge within the LNLS–Sirius user community, while also enhancing the interaction with laboratory researchers to represent the community’s scientific needs effectively.
I am applying to the LNLS User Group with the purpose of fostering integration between applied physics and life sciences, promoting the continuous improvement of technical and
scientific support, the training of new users, and the active representation of the scientific community in the laboratory’s strategic decisions”
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